Judge Eady said Friday that two Uber drivers, Yaseen Aslam and James Farrar, were working for Uber and consequently entitled to a minimum wage and holiday pay, among other rights.
Uber has long argued that its drivers are self-employed rather than workers at the firm.
On Thursday, Sean Nesbitt, partner at Taylor Wessing, said the case would be "economically significant to Uber and other gig economy and mainstream operators who are watching with interest."
Another firm operating in the gig economy, online takeaway service Deliveroo, was faced with a legal challenge of its own from delivery drivers earlier this month. Deliveroo's riders are seeking to gain employment rights including the minimum wage.
CNBC has reached out to Uber for comment.